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Protocol (a) Trypsinize hES cells as previously described (See Protocol 3.4). (b) Mechanically disperse the cells into a single-cell suspension. (c) Suspend the ES cells in methylcellulose in EB medium at 1 105 cells/ml and immediately mix the contents vigorously by vortexing. (d) The methylcellulose solution is viscous and vortexing will form bubbles within the gel, so allow 5 10 min for the bubbles to rise to the top. (e) Dispense the medium and ES cells into 6-cm Petri dishes, using a 3-ml disposable syringe attached to a 16-gauge blunt-ended needle. (f) Put the plates in the CO2 incubator.

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# put this above the $mt->view() step... $ctx =& $mt->context(); $ctx->load_filter( output , smartypants );

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Water in water out = change in storage Because calculating this equation for any one cell means updating it for its four neighbors, the calculations proceed cell by cell through the grid, iterating until the entire grid has converged There are a number of different techniques available to perform these iterative calculations (eg line-successive over-relaxation (LSOR); strongly implicit procedure (SIP); alternating direction implicit (ADI) method), each of which has its own strengths and weaknesses for certain con gurations of boundary conditions and internal parameter variations If many model runs are envisaged (for instance because the code is to be run for a large number of input data sets), it may well be worth spending some time on trial runs to identify the best solver for that particular grid Second in popularity in widely used groundwater modeling codes are nite element methods.

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When the slider isn t being use to scroll the text, the slider must be set to move in synch with the scrolling text in case the user decides to switch from button scrolling to dragging the slider. Therefore, an expression needs to be set up to increment the slider when an arrow is clicked, as shown in Listing 11-5.

As for nite difference solutions, nite elements require the subdivision of the model domain into a grid of contiguous cells One of the advantages of nite element methods over nite differences is that the cell shape can be triangular, or almost any polygonal shape; indeed, the cell shape can be allowed to vary from cell to cell In certain nite element implementations, the cells can be allowed to distort automatically during model execution (in which case they are known as deformable elements) For certain hydrogeotechnical applications, such as cross-sectional analyses of subsidence, this can be a very handy feature Other numerical methods sometimes used in groundwater ow analyses include integrated nite difference methods (which also offer the option of polygonal cells) and nite volume methods (which can be very useful in threedimensional analyses and river aquifer modeling).

FIGURE 4.21 A piezoelectric accelerometer (modern implementations use nanotechnology).

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Several numerical methods are almost wholly restricted to solving solute transport problems, such as the method of characteristics and particle tracking (also known as the random walk method; see Prickett et al 1981) Discussion of these techniques is beyond the scope of this text; the interested reader should refer to Huyakorn and Pinder (1983) and Prickett et al (1981)..

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